Journal: Cancer Cell International
Article Title: A LINC00472-encoded polypeptide impedes migration and proliferation through modulation of the HDAC2/SP1 axis in non-small cell lung cancer cells
doi: 10.1186/s12935-025-03901-z
Figure Lengend Snippet: Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) Silver staining of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) Protein-protein interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Article Snippet: Immunoprecipitated proteins were separated by 12% SDS-PAGE and visualized using the Protein Silver Staining Kit (Servicebio) according to the manufacturer’s protocol.
Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Biomarker Discovery, Silver Staining, Control, Generated